Method of photometric in vitro determination of the content of an analyte in a sample of whole blood

An analyzer for photometric determination of the content of an analyte in a sample of whole blood comprising:

• a) a radiation source;
• b) means for detecting radiation transmitted from the radiation source;
• c) a sampling device comprising:
• at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis.
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transport body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body, a detectable change in a radiation transmission characteristic of said transparent body occurs which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation transmission characteristic after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed;
• d) a sampling device station containing the sampling device;
• and
• e) means for deforming the locally transparent wall parts of the at least one measuring chamber in the sampling device in a controlled manner to reduce the volume of the at least one measuring chamber such that the sample of whole blood is substantially drained from the at least one measuring chamber, wherein the sampling device station of step (d) is arranged relative to the radiation source and the radiation detection means so that the radiation transmitted from the radiation source will pass through the at least one volume-reduced measuring chamber to the radiation detection means.

A method of photometric in vitro determination of the content of an analyte in a sample of whole blood, comprising the steps of:

• a) transferring a sample of whole blood directly from an in vivo locality to a sampling device, wherein the sampling device comprises:
• at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis,
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body a detectable change in a radiation transmission characteristic of said transparent body occurs which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation transmission characteristic after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed;
• b) placing the sampling device in a sampling device station wherein the sampling device station is arranged relative to an optical system comprised of a radiation source and a means for detecting radiation such that the sampling device is located between the radiation source and the radiation detection means;
• c) deforming the at least one measuring chamber of the sampling device in a controlled manner to reduce the volume of the at least one measuring chamber thereby substantially draining the sample of whole blood from the measuring chamber;
• d) transmitting radiation from the radiation source through the deformed, substantially drained at least one measuring chamber to the radiation detector;
• e) detecting the radiation transmitted in step d);
• and
• f) determining the analyte content of the sample of whole blood from the radiation detected in step e).

An analyzer for photometric determination of the content of an analyte in a sample of whole blood, comprising:

• a) a radiation source;
• b) means for detecting radiation transmitted from the radiation source;
• c) at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis,
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing acid analyte or reacting with said analyte such that upon contact of said analyte with said transparent body and detectable change in a radiation transmission characteristic of said transparent body occurs which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation transmission characteristic after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed,
• wherein the at least one measuring chamber is arranged relative to the radiation source and the radiation detection means so that the radiation transmitted from the radiation source will pass through the at least one measuring chamber to the radiation detection means;
• and
• d) means for deforming at least one of the locally transparent wall parts of the at least one measuring chamber in a controlled manner to reduce the volume of the at least one measuring chamber such that the sample of whole blood is substantially drained from the at least one chamber.

A method of photometric in vitro determination of the content of an analyte in a sample of whole blood, comprising the steps of:

• a) transferring a sample of whole blood to a measuring chamber which has two locally transparent wall parts located opposite one another at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the measuring chamber for photometric analysis,
• said measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing said analyte or reacting with said analyte said that upon contact of said analyte with said transparent body a detectable change in a radiation transmission characteristic of said transparent body occurs which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation transmission characteristic after displacement of the whole blood sample from said measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed.
• the measuring chamber being arranged relative to an optical system comprised of a radiation source and a means for detecting radiation such that the measuring chamber is located between the radiation source and the radiation detection means;
• b) deforming the measuring chamber in a controlled manner to reduce the volume of the measuring chamber thereby substantially draining the sample of whole blood from the measuring chamber;
• c) transmitting radiation from the radiation source through the deformed, substantially drained measuring chamber to the radiation detector;
• d) detecting the radiation transmitted in step c);
• and
• e) determining the analyte content of the sample of whole blood from the radiation detected in step d).

An analyzer for photometric determination of the content of an analyte in a sample of whole blood comprising;

• a) a radiation source;
• b) means for detecting radiation transmitted from the radiation source;
• c) a sampling device comprising:
• at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis,
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transport body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body, a detectable radiation modification is caused in said transparent body which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation modification after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed;
• d) a sampling device station containing the sampling device;
• and
• e) means for deforming the locally transparent wall parts of the at least one measuring chamber in the sampling device in a controlled manner to reduce the volume of the at least one measuring chamber such that the sample of whole blood is substantially drained from the at least one measuring chamber, wherein the sampling device station of step (d) is arranged relative to the radiation source and the radiation detection means so that the radiation transmitted from the radiation source will pass through the at least one volume-reduced measuring chamber to the radiation detection means.

A method of photometric in vitro determination of the content of an analyte in a sample of whole blood, comprising the steps of:

• a) transferring a sample of whole blood directly from in vivo locality to a sampling device, wherein the sampling device comprising:
• at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis,
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body a detectable radiation modification is caused in said transparent body which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation modification after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed;
• b) placing the sampling device in a sampling device station wherein the sampling device station is arranged relative to an optical system comprised of a radiation source and a means for detecting radiation such that the sampling device is located between the radiation source and the radiation detection means;
• c) deforming the at least one measuring chamber of the sampling device in a controlled manner to reduce the volume of the at least one measuring chamber thereby substantially draining the sample of whole blood from the measuring chamber;
• d) transmitting radiation from the radiation source through the deformed, substantially drained at least one measuring chamber to the radiation detector;
• e) detecting the radiation transmitted in step d);
• and
• f) determining the analyte content of the sample of whole blood from the radiation detected in step e).

An analyzer for photometric determination of the content of an analyte in a sample of whole blood, comprising:

• a) a radiation source;
• b) means for detecting radiation transmitted from the radiation source;
• c) at least one measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the two locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the at least one measuring chamber for photometric analysis,
• said at least one measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body a detectable radiation modification is caused in said transparent body which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation modification after displacement of the whole blood sample from said at least one measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed
• wherein the at least one measuring chamber is arranged relative to the radiation source and the radiation detection means so that the radiation transmitted from the radiation source will pass through the at least one measuring chamber to the radiation detection means;
• and
• d) means for deforming at leas tone of the locally transparent wall parts of the at least one measuring chamber in a controlled manner to reduce the volume of the at least one measuring chamber such that the sample of whole blood is substantially drained from the at least one measuring chamber.

A method of photometric in vitro determination of the content of an analyte in a sample of whole blood, comprising the steps of:

• a) transferring a sample of whole blood to a measuring chamber which has two locally transparent wall parts located opposite one another, at least one of the locally transparent wall parts being sufficiently deformable to facilitate displacement of the whole blood sample from the measuring chamber for photometric analysis,
• said measuring chamber further including a transparent body disposed between the locally transparent wall parts, the transparent body being capable of selectively absorbing said analyte or reacting with said analyte such that upon contact of said analyte with said transparent body a detectable radiation modification is caused in said transparent body. which is dependent upon the concentration of said analyte in the whole blood sample, wherein the transparent body maintains said radiation transmission characteristic after displacement of the whole blood sample from said measuring chamber so that photometric analysis of said analyte in the absence of said whole blood sample can be performed;
• the measuring chamber being arranged relative to an optical system comprised of a radiation source and a means for detecting radiation such that the measuring chamber is located between the radiation source and the radiation detection means;
• b) deforming the measuring chamber in a controlled manner to reduce the volume of the measuring chamber thereby substantially draining the sample of whole blood from the measuring chamber;
• c) transmitting radiation from the radiation source through the deformed, substantially drained measuring chamber to the radiation detector;
• d) detecting the radiation transmitted in step c);
• and
• e) determining the analyte content of the sample of whole blood from the radiation detected in step d).