Apparatus for the synthesis of saccharide compositions
AssigneeThe Trustees of the University of Pennsylvania
InventorStephen Roth
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Abstract
An apparatus for a glycosyltransferase-catalyzed synthesis of a tetrasaccharide composition of the formula gal-glcNAc-gal-B-1,4-glc from saccharide units and an acceptor moiety. The apparatus has a reactor, an inlet, an outlet, and contains two glycosyltransferases; an N-acetyl glucosaminyltransferase and a galactosyltransferase.
Claims73
An apparatus for a glycosyltransferase-catalyzed synthesis of a tetrasaccharide composition of the formula gal-glcNAc-gal-.beta.-1,4-glc from saccharide units and an acceptor moiety, said apparatus comprising reactor means having inlet means and outlet means, and containing two glycosyltransferases, an N-acetyl glucosaminyltransferase and a galactosyltransferase.
An apparatus for a glycosyltransferase-catalyzed synthesis of a tetrasaccharide composition of the formula gal-glcNac-gal-.beta.-1,4-glc, said apparatus comprising:
- (i) a reactor means containing two glycosyltransferases;
- an N-acetyl glucosaminyltransferase and a galactosyltransferase;
- (ii) inlet means for introducing lactose, UDPGlcNAc and UGPgal from a source of lactose, UDPGlcNAc and UGPgal into said reactor means such that a tetrasaccharide composition is synthesized therein;
- and
- (iii) outlet means for discharging said tetrasaccharide composition from said reactor means.
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said system comprising reactor means having inlet means and outlet means, and containing at least three different glycosyltransferases specific for a synthesis of a saccharide composition, saccharide units and an acceptor moiety, wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids, and carbohydrates;
- and wherein said reactor means comprises a plurality of reaction zones.
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said apparatus comprising reactor means having inlet means and outlet means, and containing at least three different glycosyltransferases specific for a synthesis of a saccharide composition, saccharide units and an acceptor moiety, wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids, and carbohydrates, and wherein at least one of said glycosyltransferases is immobilized onto a solid support.
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said apparatus comprising reaction means having inlet means and outlet means, and at least five different glycosyltransferases specific for a synthesis of a saccharide composition, saccharide units and an acceptor moiety, wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids, and carbohydrates.
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said apparatus comprising reaction means having inlet means and outlet means and at least four different glycosyltransferases specific for a synthesis of a saccharide composition, saccharide units and an acceptor moiety, wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids, and carbohydrates;
- and wherein at least one of said glycosyltransferases is immobilized onto a solid support.
Dependencies are inferred from the claim text.