Human nerve growth factor by recombinant technology
AssigneeGenentech, Inc.
InventorsAlane M. Gray, Axel Ullrich
Life of this patent
Estimating the term…
Abstract
The .beta.-subunit of human nerve growth factor (.beta.NGF) is prepared in essentially pure form in commercially viable quantities using recombinant DNA technology. The nucleotide sequence and vectors encoding human .beta.NGF and host cells transformed with the vectors are also provided.
Claims9
A process which comprises transforming a host cell with a replicable expression vector capable, in the host cell transformed with the vector, of expressing an isolated first DNA sequence which encodes a polypeptide comprising the human mature .beta.NGF amino acid sequence
- ______________________________________ ser ser ser his pro ile phe his arg gly glu phe ser val cys asp ser val ser val trp val gly asp lys thr thr ala thr asp ile lys gly lys glu val met val leu gly glu val asn ile asn asn ser val phe lys gln tyr phe phe glu thr lys cys arg asp pro asn pro val asp ser gly cys arg gly ile asp ser lys his trp asn ser tyr cys thr thr thr his thr phe val lys ala leu thr met asp gly lys gln ala ala trp arg phe ile arg ile asp thr ala cys val cys val leu ser arg lys ala val arg ______________________________________
- operably linked with a second DNA sequence capable of effecting expression of the first DNA sequence in the host cell transformed with the operably linked DNA sequences.
A method of using a host cell transformed with a replicable expression vector capable of expressing an isolated DNA sequence encoding the human mature .beta.NGF amino acid sequence
- ______________________________________ ser ser ser his pro ile phe his arg gly glu phe ser val cys asp ser val ser val trp val gly asp lys thr thr ala thr asp ile lys gly lys glu val met val leu gly glu val asn ile asn asn ser val phe lys gln tyr phe phe glu thr lys cys arg asp pro asn pro val asp ser gly cys arg gly ile asp ser lys his trp asn ser tyr cys thr thr thr his thr phe val lys ala leu thr met asp gly lys gln ala ala trp arg phe ile arg ile asp thr ala cys val cys val leu ser arg lys ala val arg ______________________________________
- which comprises culturing the host cell under conditions such that the expression vector is replicated.
A method of using a host cell transformed with a replicable expression vector capable of expressing an isolated DNA sequence encoding the human mature .beta.NGF amino acid sequence
- ______________________________________ ser ser ser his pro ile phe his arg gly glu phe ser val cys asp ser val ser val trp val gly asp lys thr thr ala thr asp ile lys gly lys glu val met val leu gly glu val asn ile asn asn ser val phe lys gln tyr phe phe glu thr lys cys arg asp pro asn pro val asp ser gly cys arg gly ile asp ser lys his trp asn ser tyr cys thr thr thr his thr phe val lys ala leu thr met asp gly lys gln ala ala trp arg phe ile arg ile asp thr ala cys val cys val leu ser arg lys ala val arg ______________________________________
- which comprises culturing the host cell under conditions such that human mature .beta.NGF accumulates in the culture.
Dependencies are inferred from the claim text.