Method for the detection of malignant diseases
AssigneeBoehringer Mannheim GmbH
InventorsHeinz Bodenmuller, Andreas Dessauer
Life of this patent
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Abstract
In order to aid in the detection of malignant diseases the sample of a body fluid is incubated with at least two receptors R.sub.1 and R.sub.2 in which a signal change is produced by binding of at least the receptors R.sub.1 and R.sub.2 to the substance to be detected in the sample solution and in which one of the two receptors contains a monoclonal antibody which binds to the amino acid sequence 311 to 335 of cytokeratin 19 and the other receptor contains a monoclonal antibody which binds to the amino acid sequence 346 to 359 of cytokeratin 19 and the signal change in the sample caused by the binding is determined.
Claims15
Reagent to aid in the detection of malignant diseases, comprising at least two receptors R1 and R2, wherein one of the receptors contains a monoclonal antibody which specifically binds to the sequence 311 to 335 of cytokeratin 19 and other receptor contains a monoclonal antibody which specifically binds to the sequence 346 to 359 of cytokeratin 19.
The monoclonal antibody produced by the cell line ECACC 89112803.
The monoclonal antibody produced by the cell line ECACC 89112804.
Cell line ECACC 89112803.
Cell line ECACC 89112804.
Method for screening for malignant diseases, comprising the steps of:
- incubating a sample of a body fluid with at least two receptors R1 and R2 in a liquid phase, wherein one of the two receptors contains a monoclonal antibody which specifically binds to the amino acid sequence 311 to 335 of cytokeratin 19 and the other receptor contains a monoclonal antibody which specifically binds to the amino acid sequence 346 to 359 of cytokeratin 19, to produce a signal change by binding of the receptors R1 and R2 to the substance to be detected in the sample solution, and
- detecting the signal change in the sample caused by the binding as an indication of the presence of a malignant disease.
Dependencies are inferred from the claim text.