US 5,288,606 A · Reagent for specific determination of fructosamine

Abstract

The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto. The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.

Claims20

Dependent claims

Reagent useful in determining serum fructosamine in a sample of blood or a sample derived from blood with avoidance of the protein matrix effect comprising:

(a) a first agent comprising an enzymatic oxidizing agent and a detergent selected from the group consisting of anionic, non-ionic, and cationic detergents;
(b) a second agent comprising a tetrazolium salt which reacts with fructosamine to form a color;
and
(c) a third agent which is a buffer at a pH of from about 10.5 to about 12.5.

1 more element

Dependencies are inferred from the claim text.

Reagent for specific determination of fructosamine

Life of this patent

Reagent for specific determination of fructosamine

Life of this patent

Abstract

Claims20