Method of forming planar membrane
AssigneeCanon Kabushiki Kaisha
InventorsTsunehiro Kanno, Kinya Kato, Harumi Iwashita, Junji Ohyama, Nobuko Yamamoto, Masanori Sakuranaga
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Abstract
A method of forming a lipid planar membrane of a bilayer membrane composed of lipid and protein comprises the step of immersing a substrate, which has a long-chain hydrocarbon group at least on the surface of one side thereof, in a suspension of liposome or proteoliposome, respectively. The long-chain hydrocarbon group is introduced into the substrate by bonding a long-chain alkylsilane coupling agent or a long-chain alkyltitanate coupling agent.
Claims6
A method of forming a lipid planar bilayer membrane, comprising the steps of selecting a liquid phase comprising a suspension of liposome;
- and
- immersing a substrate having on the surface of at least one side thereof a molecular layer of long-chain hydrocarbon groups in said liquid phase, whereby said liposome is cleaved, on the surface of said molecular layer in said liquid phase and is disposed as a monolayer on said molecular layer.
A method of forming a bilayer membrane composed of lipid and protein, comprising the steps of immersing a substrate having on the surface of at least one side thereof a molecular layer of long-chain hydrocarbon groups;
- and
- immersing said substrate in a liquid phase comprising a suspension of proteoliposome whereby said proteoliposome is cleaved, on the surface of said molecular layer in said liquid phase and is disposed as a monolayer on said molecular layer.
Dependencies are inferred from the claim text.