US 5,288,477 A · Method for prognosticating response to cancer therapy

Abstract

A method for prognosticating the effectiveness of a chemotherapy using monoclonal antibodies and ligand molecules. The putative anti-cancer agent has binding specificity for a oncogenic receptor molecule on the membrane of a cancer cell, such as HER-2/neu. When the putative agent binds to the oncogenic receptor, the receptor translocates from the membrane to the cytoplasm or perinucleus of the cancer cell, accompanied by a transient increase in the total cellular content of the receptor, and results in terminal cell differentiation. The efficacy of the agent in vivo can be determined in vitro by treatment of biopsied cancer cells with the agent and subsequent examination of the cells for evidence of terminal cell differentiation. Such evidence includes morphological change, reduction in cell growth, or production of chemicals associated with the mature phenotype. Additionally, treated cells may be examined with immunohistochemicals specific for the oncogenic receptor, to determine translocation of the receptor from the membrane to the cytoplasm or perinucleus. Quantification of receptor levels in treated cells by measuring optical densities after staining can be used to determine translocation, as well as a transient increase in total cellular content of the receptor.

Claims17

Dependent claims

A method for determining/prognosticating the effectiveness of a therapeutic agent in the treatment of a cancer by measuring the ability of the therapeutic agent to induce terminal cell differentiation wherein malignant cells of the cancer overexpress an oncogene product, the method comprises the steps of:

(a) obtaining from a human having such a cancer a biopsy comprising viable malignant cells which overexpress at least one oncogene product and dividing the same into first and second portions;
(b) treating the first portion comprising viable cells with a sufficient quantity of a composition comprising at least one compound having specific binding affinity for the oncogene product and contacting the second portion with a composition which is devoid of the at least one compound having specific binding affinity for the oncogene product;
(c) maintaining the first and second portions in a physiologically acceptable medium for an amount of time sufficient to induce maturation in the viable malignant cells of said first portion:
and
(d) comparing the percentage of cells in the first portion which exhibit markers of terminal cell differentiation to the percentage of cells in the second portion which exhibit markers of terminal cell differentiation wherein effectiveness of treatment correlates with the degree of terminal cell differentiation.

2 more elements

Dependencies are inferred from the claim text.

Method for prognosticating response to cancer therapy

Life of this patent

Method for prognosticating response to cancer therapy

Life of this patent

Abstract

Claims17