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US 5,286,641 AGranted
In vitro culture method for a fertilized ovum of a hen
AssigneeDirector General, National Institute of Animal Industry
InventorMitsuru Naito
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Abstract
A fertilized ovum is recovered from an upper portion of a magnum of an oviduct of a dead hen 60 to 80 minutes after oviposition. The fertilized ovum is placed in a container containing a thin albumen. A yolk of the ovum is covered with a sheet of gauze, and the ovum is cultured up to the blastoderm stage (stage X) in the container which is covered with a sheet of plastic film that is held by a rubber band.
Claims4
An in vitro culture method for a fertilized ovum of a hen comprising:
- recovering a fertilized ovum from an upper portion of a magnum of an oviduct of a dead hen 60 to 80 minutes after oviposition;
- providing thin albumen in a container having sufficient capacity to hold said fertilized ovum and a sufficient amount of said thin albumen to partially submerge said fertilized ovum;
- placing said fertilized ovum in said container and partially submerging said fertilized ovum in said thin albumen whereby effectively contacting said fertilized ovum with said thin albumen in said container;
- covering a yolk of the ovum with a sheet of gauze which is in effective contact with said thin albumen and with said yolk;
- and
- culturing the ovum so covered with said gauze up to the blastoderm stage.
3 more elements
Dependencies are inferred from the claim text.