US 5,286,484 A · Nucleotide sequence coding for an outer membrane protein from Neisseria meningitidis and use of said protein in vaccine preparations

Nucleotide sequence coding for an outer membrane protein from Neisseria meningitidis and use of said protein in vaccine preparations

AssigneeCentro de Ingenieria Genetica y Biotecnologia

InventorsSilva Rodriquez, Selman H. Sosa, Guillen Nieto, Saturnino H. Martinez, Julio R. F. Maso, Lidia I. N. Perez, Juan M. Grillo, Vivian M. Cordova, Sonia G. Blanco, Beatriz T. Santos, Jesus A. del Valle Rosales, Evelin C. Menendez, Anabel A. Acosta, Edelgis C. Rodriquez, Silian C. Leon, Alexis M. Lasa

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Abstract

The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64,000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.

Claims12

Dependent claims

A recombinant polynucleotide, comprising a nucleotide sequence coding for a protein P64k of Neisseria meningitidis, said protein P64k essentially having the amino acid sequence shown in SEQ ID NO:1.

Dependencies are inferred from the claim text.