Process for obtaining glycolipids and phospholipids

A process for obtaining glycolipids and phospholipids comprising treating lactose-free buttermilk with a polar solvent to obtain a solution containing neutral lipids and a residue containing complex lipids, eliminating the polar solvent from the residue, treating the residue with a slightly polar solvent to obtain a solution containing complex lipids, eliminating the slightly polar solvent to obtain a concentrate, eluting the concentrate through a column containing silica gel to separate complex lipids from remaining neutral lipids, passing the complex lipids over a gel of copolymerized and crosslinked dihydroxyborylanilinomethacrylic acid and 1,4-butanediol dimethacrylate so that glycolipids are adsorbed on the gel and phospholipids pass through the gel, and collecting phospholipids and glycolipids.

A process for obtaining glycolipids and phospholipids comprising treating lactose-free buttermilk with a slightly polar solvent to form a solution and a residue, separating the residue from the solution, eliminating solvent from the solution to obtain a concentrate, treating the concentrate with a polar solvent, collecting an insoluble fraction from the polar solvent, eliminating traces of polar solvent to obtain a concentrate, separating complex lipids from the concentrate by adsorption chromatography on silica gel, passing the complex lipids over a gel of copolymerized and crosslinked dihydroxyborylanilinomethacrylic acid and 1,4-butanediol dimethacrylate so that glycolipids are adsorbed on the gel and phospholipids pass through the gel, and collecting phospholipids and glycolipids.