Apparatus and methods for electroporation and electrofusion
OwnerRobert E. Schmuklerinventor
Life of this patent
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Abstract
An insulating film in a container has pores whose diameter is smaller than the diameter of a first type of cells in the container. The first type of cells are trapped in the pores, so that when an electric field is applied to the container, electroporation of the first type of cells occurs. If a second type of cells, smaller than the first type of cells, are also trapped in the pores, electrofusion will occur between the first and second types of cells trapped in the same pores.
Claims8
An apparatus for electroporation of a first type of cells comprising:
- a container;
- an insulating film, penetrated by pores, dividing the container into two sections;
- a first type of cells in a solution in one of the sections, the diameter of the first type of cells being larger than the diameter of the pores;
- means for causing the first type of cells to become trapped in different ones of the pores such that a portion of each of the trapped first type of cells extends into a different one of the pores;
- and
- means for applying a low voltage electric field of less than 25 volts to the container to pass through the two sections and the pores to create a substantially larger voltage gradient across the trapped cells than across the remainder of the container to cause electroporation of the trapped first type of cells at the portions which extend into the pores.
An apparatus for electrofusion of a first type of with a second type of cells which are smaller than the first type of cells comprising:
- a container containing an insulating film, penetrated by pores, which divides the container into two sections;
- a first type of cells in a solution in one section of the container, the diameter of the first type of cells being larger than the diameter of the pores;
- means for causing the first type of cells to become trapped in different ones of the pores such that a portion of each of the trapped first type of cells extends into a different one of the pores;
- a second type of cells in the other section of the container;
- means for causing trapped ones of the second type of cells to enter from a second side of the insulating film ones of the pores of the insulating film into which trapped first type of cells extend and to contact trapped first type of cells;
- and
- means for applying an electric field to pass through both of sections of the container and the pores to cause electroporation of the first type of cells at the portions which are trapped in the pores and to cause electroporation of the second type of cells in the pores, and then to cause electrofusion of the trapped first type of cells and the trapped second type of cells which are in the same ones of the pores and in contact with each other.
An apparatus for electroporation of a first type of cells comprising:
- a container;
- an insulating film, penetrated by pores, dividing the container into two sections;
- a first type of cells in a solution in one of the sections, the diameter of the first type of cells being larger than the diameter of the pores;
- means for causing the first type of cells to become trapped in different ones of the pores such that a portion of each of the trapped first type of cells extends into a different one of the pores, the means for causing the first type of cells to become trapped including
- lines for introducing a pressure into the container to draw the first type of cells into the pores;
- and
- means for applying a low voltage electric field to the container to pass through the two sections and the pores to create a substantially larger voltage gradient across the trapped first type of cells than across the remainder of the container, thereby to cause electroporation of the trapped first type of cells at the portions which extend into the pores.
Dependencies are inferred from the claim text.