Preparation of an activated polymer ester for protein conjugation
AssigneeCetus Oncology Corporation
InventorDanute E. Nitecki
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Abstract
The present invention is a process for preparing an activated ester of polyethylene glycol or a polyoxyethylated polyol. After the activated ester is prepared, it can be reacted with a protein to form a polymer/protein conjugate. Conjugation with a polymer can reduce the protein's immunogenicity, increase its solubility, and increase its circulating in vivo half-life. Preferred proteins are IL-2, CSFs, and interferons.
Claims20
A method for producing an activated ester of polyethylene glycol (PEG) or a polyoxyethylated polyol, comprising:
- a) contacting PEG or a polyoxyethylated polyol, which has at least one hydroxyl group, with disuccinimidylcarbonate (DSC) under the appropriate reaction conditions to form a PEG or a polyoxyethylated polyol active ester.
A method in accordance with claim. 1, wherein the PEG or the polyoxyethylated polyol has an average molecular weight between 3,000 and 12,000.
Dependencies are inferred from the claim text.