Modified avidin-biotin technique
AssigneeThe Trustees of the University of Pennsylvania
InventorsJohn Q. Trojanowski, Virginia M-Y. Lee
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Abstract
A method for preparing an antigen specific probe is provided by incubating a primary antigen specific monoclonal antibody with a biotinylated secondary antibody to produce a complex of the primary and secondary antibodies. The staining pattern produced by these probes reflects the specificity of the monoclonal antibody in the complex and the labeling of irrelevant, endogenous immunoglobulins is reduced substantially. This novel, indirect immunohistochemical method can be used to study normal and diseased tissues using a variety of monoclonal antibodies.
Claims8
A method for preparing an antigen specific complex comprising incubating a selected primary antigen specific monoclonal antibody which is not bound to said primary antigen with a selected biotinylated secondary antibody to produce a complex of the primary and secondary antibodies.
Dependencies are inferred from the claim text.