Merocyanine and nitro or nitroso substituted polyhalogenated phenolsulfonephthaleins as protein indicators in biological samples
AssigneeMiles Inc.
InventorsPaul F. Corey, Angela A. Michaels, Ronald G. Sommer
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Abstract
The present invention provides an analytical test strip for the detection of protein in a biological sample including a novel combination of protein error indicators. The invention includes, in combination, a partially halogenated phenolsulfonephthalein protein error indicator having nitro or nitroso substituent groups in the B and/or C rings and a merocyanine protein error indicator.
Claims18
An analytical test strip for the detection of protein in a biological sample comprising an absorbent carrier impregnated with a phenolsulfonephthalein protein error indicator and a merocyanine protein error indicator, the phenolsulfonephthalein protein error indicator is the compound:
- ##STR11## wherein:
- X is --I, --Br or --Cl;
- Y is --NO.sub.2 or --NO;
- and
- Z is --I, --Br or --Cl;
- and the merocyanine protein error indicator is in a concentration sufficient to produce a colorimetric change in the presence of protein wherein the compound is ##STR12## wherein:
- m is an integer from 1 to 6;
- Q is --Br, --I or Cl;
- T is --SO.sub.3 .theta. or --H;
- and
- R is S, Se, O or C(C.sub.n H.sub.2n+1).sub.2,
- wherein:
- n is an integer from 1 to 6.
An analytical test strip for the detection of protein in a biological sample comprising an absorbent carrier impregnated with a phenolsulfonephthalein protein error indicator and a merocyanine protein error indicator, the phenolsulfonephthalein protein error indicator is the compound:
- ##STR13## wherein:
- X is --I, --Br or --Cl;
- Y' is --NO.sub.2 or --NO;
- Y" is --I, --Br or --Cl;
- and
- Z is --I, --Br or --Cl;
- and the merocyanine protein error indicator is in a concentration sufficient to produce a colorimetric change in the presence of protein wherein the compound is ##STR14## wherein:
- m is an integer from 1 to 6;
- Q is --Br, --I or Cl;
- T is --SO.sub.3 .theta. or --H;
- and
- R is S, Se, O or C(C.sub.n H.sub.2n+1).sub.2,
- wherein:
- n is an integer from 1 to 6.
A method for the detection of protein in a biological sample, the method comprising the steps of:
- (a) wetting an analytical test strip with the biological sample, the test strip including an absorbent carrier impregnated with a phenolsulfonephthalein protein error indicator and a merocyanine protein error indicator, the phenolsulfonephthalein protein error indicator is the compound:
- ##STR15## wherein:
- X is --I, --Br or --Cl;
- Y is --NO.sub.2 or --NO;
- and
- Z is --I, --Br or --Cl;
- and the merocyanine protein error indicator is in a concentration sufficient to produce a colorimetric change in the presence of protein wherein the compound is ##STR16## wherein:
- m is an integer from 1 to 6;
- Q is --Br, --I or Cl;
- T is --SO.sub.3 .theta. or --H;
- and
- R is S, Se, O or C(C.sub.n H.sub.2n+1).sub.2,
- wherein:
- n is an integer from 1 to 6;
- (b) observing and recording any color change of the test strip, wherein a color change is indicative of protein in the biological sample.
A method for the detection of protein in a biological sample, the method comprising the steps of:
- (a) wetting an analytical test strip with the biological sample, the test strip including an absorbent carrier impregnated with a phenolsulfonephthalein protein error indicator and a merocyanine protein error indicator, the phenolsulfonephthalein protein error indicator is the compound:
- ##STR17## wherein:
- X is --I, --Br or --Cl;
- Y' is --NO.sub.2 or --NO;
- Y" is --I, --Br, or --Cl;
- and
- Z is --I, --Br or --Cl;
- and the merocyanine protein error indicator is in a concentration sufficient to produce a colorimetric change in the presence of protein wherein the compound is ##STR18## wherein m is an integer from 1 to 6;
- Q is --Br, --I or Cl;
- T is --SO.sub.3 .theta. or --H;
- and
- R is S, Se, O or C(C.sub.n H.sub.2n+1).sub.2,
- wherein:
- n is an integer from 1 to 6;
- and
- (b) observing and recording any color change of the test strip, wherein a color change is indicative of protein in the biological sample.
Dependencies are inferred from the claim text.