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AssigneeThe Trustees of Columbia University in the City of New York
InventorsArthur Bank, Dina G. Markowitz, Stephen P. Goff
Estimating the term…
The invention relates to a mammalian cell line useful for retroviral packaging comprising two plasmids, the first such plasmid comprising in 5' to 3' order: a DNA sequence comprising a 5' long terminal repeat (LTR); a mutated .psi. packaging sequence; a DNA sequence comprising the encoding portion of the Moloney murine leukemia virus (MULV) env gene; and the second such plasmid comprising in 5' to 3' order: a DNA sequence comprising a 5' long terminal repeat (LTR); a mutated .psi. packaging sequence; a DNA sequence comprising the encoding portion of the Moloney murine leukemia virus (MULV) gag and pol genes; a selectable marker; and an origin of replication. The invention also relates to processes for preparing a producer cell line useful for transferring a gene of interest into recipient mammalian cells in vitro.
The ecotropic cell line designated GP+E-86 (ATCC Accession No. CRL 9642).
The amphotropic cell line designated GP+envAm-12 (ATCC Accession No. CRL 9641).
A process for generating a producer cell line useful for transferring a gene of interest into recipient mammalian cells comprising transfecting cells of the cell line of claim 31 or 32 with a vector plasmid which comprises a psi packaging sequence, a gene associated with a selectable or identifiable phenotypic trait which is manifested when the plasmid is present in a transfected cell, and a gene of interest.
A method for transferring a gene of interest into recipient mammalian cells comprising:
Dependencies are inferred from the claim text.