Recombinant human cytomegalovirus containing foreign gene
AssigneeSyntro Corporation
InventorsLisa J. Hock, Mark D. Cochran, Richard D. Macdonald
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Abstract
The present invention provides a virus comprising a DNA sequence essential for replication of a human cytomegalovirus and at least one foreign DNA sequence adapted for expression in a host. The foreign DNA sequence may encode a human immunodeficiency virus anti-sense mRNA sequence or an antigenic polypeptide, e.g., a malarial surface antigen. Also provided are therapeutic compositions and vaccines which comprise the novel viruses of the present invention.
Claims9
Recombinant cytomegalovirus comprising (i) the genomic DNA of Towne strain human cytomegalovirus;
- (ii) a foreign DNA sequence inserted within the 5.4 Kb BamHI region shown in FIG. 1 or within the 5.4 Kb XhoI region shown in FIG. 2 of Towne strain human cytomegalovirus genome;
- and (iii) a promoter sequence attached to the foreign DNA at location upstream of the said foreign DNA, the promoter sequence being the endogenous human cytomegalovirus major immediate early promoter or a foreign herpesvirus promoter.
A plasmid designated pSY1157.
A plasmid designated pSY1159.
Dependencies are inferred from the claim text.