Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
AssigneeGeorgetown University
InventorsMark E. Smulson, Kishor Bhatia, Konrad Huppi
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Abstract
The invention relates to a method for detecting the presence of a DNA polymorphism associated with predisposition for certain cancers in a human. This method involves analyzing the human chromosome 13 using a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein the probe is capable of identifying the DNA polymorphism.
Claims20
A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt's lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
- (a) obtaining a biological sample having nucleated cells from a human;
- (b) isolating said nucleated cells from said biological sample;
- (c) isolating DNA from said nucleated cells;
- (d) digesting said DNA with the restriction enzyme HindIII to obtain DNA fragments;
- (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments;
- (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly(ADP-ribose) polymerase wherein said probe will also hybridize to at least one DNA fragment of about 2.5 to about 2.7 kilobases in a human with a predisposition for cancer.
A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt's lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
- (a) obtaining a biological sample having nucleated cells from a human;
- (b) isolating said nucleated cells from said biological sample;
- (c) isolating DNA from said nucleated cells;
- (d) digesting said DNA with the restriction enzyme PstI to obtain DNA fragments;
- (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments;
- (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly(ADP-ribose) polymerase wherein said probe will also hybridize to a DNA fragment of about 5.7 kilobases to about 6.1 kilobases in a human with a predisposition for cancer.
A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt's lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
- (a) obtaining a biological sample having nucleated cells from a human;
- (b) isolating said nucleated cells from said biological sample;
- (c) isolating DNA from said nucleated cells;
- (d) digesting said DNA with the restriction enzyme EcoR1 to obtain DNA fragments;
- (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments;
- (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein said probe will also hybridize to a DNA fragment of about 5.1 kilobases in a human with a predisposition for cancer.
The method of any of claims 1 to 8 wherein said biological sample is selected from the group consisting of blood, tissue and sperm.
A method for detecting susceptibility for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt's lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
- identifying the presence of a DNA polymorphism on chromosome 13 associated with susceptibility for cancer using a hybridisation probe which will hybridize to the gene for human poly (ADP-ribose) polymerase or closely linked sequences on chromosome 13 wherein the probe is capable of identifying said DNA polymorphism.
A restriction fragment length polymorphic probe comprising nucleic acid sequences which will hybridize to the gene for poly (ADP-ribose) polymerase and will also detect a DNA polymorphism associated with a susceptibility for cancer on human chromosome 13.
Dependencies are inferred from the claim text.