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AssigneeSepracor Inc.
InventorsPierre Girot, Egisto Boschetti
Estimating the term…
This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous mineral oxide supports are disclosed that are stabilized against leaching under harsh environmental conditions by the application of a thin, protective polymeric coating atop their porous surfaces and that are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by the use of a passivation mixture comprising a main monomer, a passivating monomer that is different from the main monomer, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction between biomolecules and the protective polymeric coating on the surfaces of the mineral oxide matrix.
A passivated porous support comprising (i) a porous mineral oxide matrix having interior and exterior surfaces substantially covered by a thin, protective polymer surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interaction with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating such that on polymerization of said mixture said innate groups of said coating become deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
The passivated porous support of claim I8 in which said polymerization of said passivation mixture is effected by thermal energy.
A passivated porous support comprising (i) a porous silica matrix having interior and exterior surfaces substantially covered by a thin, protective polystyrene surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interactions with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating for a sufficient period of time such that on polymerization of said mixture said hydrophobic groups of said coating become substantially covered and deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
Dependencies are inferred from the claim text.