US 5,266,491 A · DNA fragment and expression plasmid containing the DNA fragment

Abstract

Novel expression plasmids containing SV40 replication origin and a DNA fragment having a promoter region for a human polypeptide chain elongation factor-1.alpha. gene. The expression plasmids have high applicability to a wide range of host cells with high expression efficiency in transient expression systems.

Claims7

Dependent claims

An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:4):

##STR5##

Dependencies are inferred from the claim text.

Facts

Application

07/757,536Filed 11 Sep 1991 · continuation in part

Priority

JP 1-6170214 Mar 1989

Granted

30 Nov 1993

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C07K 14/47
From vertebrates: from mammals
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells
C12N 2830/00
Vector systems having a special element relevant for transcription
C12N 2830/38
Vector systems having a special element relevant for transcription: being a stuffer

Abstract

Claims7

Dependent claims

An essentially pure DNA fragment which is represented by the following sequence (SEQ ID NO:4):

##STR5##

Dependencies are inferred from the claim text.

DNA fragment and expression plasmid containing the DNA fragment

Life of this patent

Abstract

Claims7

DNA fragment and expression plasmid containing the DNA fragment

Life of this patent

Abstract

Claims7