Fibrous matrix for in vitro cell cultivation
AssigneeYeda Research and Development Co. Ltd.
InventorsMartin Sussman, Zvi Bohak, Avinoam Kadouri
Life of this patent
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Abstract
There is provided a matrix and cultivation system for anchorage dependent cells. The matrix is characterized by a substantially increased available effective surface area for cell attachment which is attained by resorting to the use of a fiber network or open-pore foams with suitable pore size. Attachment of the cells can be enhanced by modifying the surface of the substrate. One embodiment comprises a matrix in particle or flake form.
Claims24
A substrate in the form of a sheet for use in cell cultivation in vitro comprising a nonwoven fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable three-dimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns, said substrate having a surface area of from about 1 mm.sup.2.
A system for cell cultivation in vitro comprising a substrate in the form of a sheet comprising a nonwoven fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable three-dimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns.
The system for cell cultivation in vitro comprising a nonwoven plurality of substrates, each in the form of a sheet, each substrate comprising a fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable threedimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns, wherein said sheets are stacked with spacing means between adjacent matrix sheets.
A system for cell cultivation in vitro comprising a plurality of substrates each in the form of a sheet, each substrate comprising a nonwoven fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable threedimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns, said system comprising layers of said matrix sheets between which flattened permeable or microporous tubular conduits are interleaved, said layers of interleaved structure being arranged in a stack or a spiral.
A system for cell cultivation in vitro comprising a plurality of substrates each in the form of a sheet, each substrate comprising a nonwoven fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable threedimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns, each said matrix being in the form of a rectangle which is attached as a liner inside the surface of a container to be used for cell cultivation.
A system for cell cultivation in vitro comprising a culture vessel containing a bed of randomly packed substrates, each substrate comprising a fibrous matrix bonded to a porous support sheet, each said matrix comprising a physiologically acceptable three-dimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns.
A substrate in the form of a sheet for use in cell cultivation in vitro comprising a nonwoven fibrous matrix bonded to a porous support sheet, said matrix comprising a physiologically acceptable three-dimensional network of fibers in the form of a sheet having a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns, the overall height of the matrix being from 50 microns to 500 microns, said matrix having a surface area of from about 1 mm.sup.2 to about 700 mm.sup.2 and a height of from about 50 microns to about 250 microns, and said substrate being in a shape selected from the group consisting of squares, rings, discs, and cruciforms.
Dependencies are inferred from the claim text.