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AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsMichael D. Tomalski, Lois K. Miller
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Genes encoding insect-specific paralytic neurotoxins, particularly those of insect-parasitic mites, including Pyemotes, are described. Recombinant DNA molecules in which the neurotoxin coding sequences are placed under the control of heterologous promoters are also described. Such molecules are useful for the development of biological insect control agents which produce insect-toxic levels of the neurotoxin. Specifically described are genetically altered baculoviruses which produce insect-specific paralytic neurotoxins and which display improved toxic effect on insects. Insect-toxic compositions are also provided. Methods of insect control using these neurotoxin genes, methods for production of neurotoxins in cells, and methods of production of insect control agents are described.
An isolated and purified recombinant DNA molecule comprising a first nucleic acid sequence and a second nucleic acid sequence, said first nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said nucleic acid sequence has at least 70% nucleotide sequence homology to the nucleotide sequence encoding an insect-specific paralytic neurotoxin as shown in SEQ ID NO:4 from nucleotide 129 to nucleotide 884, and said second nucleic acid sequence being selected from the group consisting of a virus vector, a bacteriophage vector and a plasmid vector, wherein said first nucleic acid is covalencly linked to said second nucleic acid sequence.
An isolated and purified recombinant DNA molecule comprising a first nucleic acid sequence and a second nucleic acid sequence, said first nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, said neurotoxin having an amino acid sequence with at least about 83% amino acid sequence similarity with an amino acid sequence of an insect-specific paralytic neurotoxin of an insect-predacious mite as in SEQ ID NO:7, and said nucleic acid sequence being selected from the group consisting of a virus vector, a bacteriophage vector and a plasmid vector.
An isolated and purified recombinant DNA molecule comprising a first nucleic acid sequence and a second nucleic acid sequence, said first nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, said nucleic acid sequence having at least about 70% nucleic acid sequence homology with a nucleic acid sequence as in SEQ ID NO:6 from nucleotide 119 to nucleotide 985, and said second nucleic acid sequence being selected from the group consisting of a virus vector, a bacteriophage vector and a plasmid vector.
A baculovirus insect control agent which has been genetically engineered to contain and express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect predacious mite, said neurotoxin having at least 83% amino acid sequence identity with an amino acid sequence as in SEQ ID NO;5 from an aspartate at amino acid 1 to a cysteine at amino acid 252.
An isolated and purified recombinant DNA molecule comprising a first nucleic acid sequence and a second nucleic acid sequence, said first nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said encoded neurotoxin has at least about 83% sequence identity with an amino acid sequence as shown in SEQ ID NO:5 from an aspartate at amino acid 1 to a cysteine at amino acid 252, and a second nucleic acid sequence, said second nucleic acid sequence selected from the group of consisting of a virus vector, a bacteriophage vector and a plasmid vector, wherein said first nucleic acid sequence is covalently jointed to said second nucleic acid sequence.
A baculovirus insect control agent which has been genetically engineered to contain and express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect predacious mite, said nucleic acid sequence having at least 70% nucleic acid sequence homology with a nucleic acid sequence as in SEQ ID NO:4, from nucleotide 129 to nucleotide 884.
A baculovirus insect control agent which has been genetically engineered to contain and express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, where in said encoded neurotoxin has at least about 83% amino acid sequence identity with an amino acid sequence as in SEQ ID NO:7.
A baculovirus insect control agent which has been genetically engineered to contain and express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said nucleic acid sequence has at least about 70% nucleic acid sequence homology with a nucleic acid sequence as in SEQ ID NO:6 from nucleotide 119 to nucleotide 985.
An insect-toxin composition comprising an amount of a baculovirus effective for causing a toxic effect on a target insect, which baculovirus has been genetically modified to express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said nucleic acid sequence has at least about 70% nucleotide sequence homology to a nucleic acid sequence as in SEQ ID NO:4 from nucleotide 129 to nucleotide 884, said composition further comprising an agriculturally acceptable carrier.
An insect-toxic composition comprising an amount of a baculovirus effective for causing a toxic effect on a target insect, which baculovirus has been genetically modified to express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said encoded neurotoxin has at least about 83% amino acid sequence identity with an amino acid sequence as in SEQ ID NO:5 from amino acid 1 to amino acid 252, said composition further comprising an agriculturally acceptable carrier.
An insect-toxic composition comprising an amount of a baculovirus effective for causing a toxic effect on a target insect, which baculovirus has been genetically modified to express an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein said neurotoxin has at least about 83% amino acid sequence identity with an amino acid sequence as in SEQ ID NO:7, said composition further comprising an agriculturally acceptable carrier.
An insect-toxic composition comprising an amount of a baculovirus effective for causing a toxic effect on a target insect, which baculovirus has been genetically modified to express a nucleic acid sequence encoding an insect-specific paralytic neurotoxin of an insect-predacious mite, wherein nucleic acid sequence has at least about 70% nucleic acid sequence homology to a nucleic acid sequence as in SEQ ID NO:6 from nucleotide 119 to nucleotide 985, said composition further comprising an agriculturally acceptable carrier.
A method for the production of an insect-specific paralytic neurotoxin of an insect-predacious mite in a host cell, wherein a nucleic acid sequence encoding said neurotoxin has at least about 70% nucleotide sequence homology with a nucleotide sequence as given in SEQ ID NO:4, from nucleotide 129 to nucleotide 884, said method comprising the steps of:
Dependencies are inferred from the claim text.