US 5,262,325 A · Method for the enzymatic neutralization of heparin

Abstract

A heparinase formulation derived from Flavobacterium heparinum which meets all requirements for a clinical reagent that can eliminate heparin interference of normal blood function has been developed. The heparinase, derived from Flavobacterium heparinum, is free of a component that inhibits coagulation. It is stable under normal manufacturing, shipping and clinical storage conditions for at least one year. The heparinase in useful in vitro to eliminate the interference in hematological assays due to the presence of heparin. The heparinase is also useful for the in vivo neutralization of heparin during surgical procedures. Advantages of this preparation are that it achieves neutralization faster and more completely than previously available compositions and is stable for long periods of time.

Claims11

Dependent claims

A method to eliminate the physiological effects of heparin on blood components in a mixture of blood components and heparin comprising

contacting blood components containing heparin with a bacterial heparinase preparation,
wherein the heparinase preparation is free of an anticoagulant component prior to mixing of the heparinase preparation with the heparin,
wherein the anticoagulant component does not bind to a polysulfated resin at pH 7.0, conductivity between 3 and 12 mmhos, and the heparinase does bind to a polysulfated resin at pH 7.0, conductivity between 3 and 12 mmhos, and
wherein the heparinase preparation free of an anticoagulant component has an optimal activity at pH=6.5 to 7.0;
salt concentration=0.1M, and 37.degree. C.

2 more elements

Dependencies are inferred from the claim text.

Method for the enzymatic neutralization of heparin

Life of this patent

Method for the enzymatic neutralization of heparin

Life of this patent

Abstract

Claims11