Single polypeptide chain binding molecules
AssigneeEnzon, Inc.
InventorsRobert C. Ladner, Robert E. Bird, Karl Hardman
Life of this patent
Estimating the term…
Abstract
The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Claims20
A single chain polypeptide having binding affinity for a given antigen, said polypeptide comprising:
- (a) a first polypeptide comprising the antigen binding portion of the light chain variable region of an antibody;
- (b) a second polypeptide comprising the antigen binding portion of the heavy chain variable region of an antibody;
- and
- (c) at least one peptide liner linking said first and second polypeptides (a) and (b) into a single chain polypeptide having binding affinity for said given antigen.
A single chain polypeptide having binding affinity for a given antigen, said polypeptide comprising:
- (a) a first polypeptide comprising the antigen binding portion of the light chain variable region of an antibody;
- (b) a second polypeptide comprising the antigen binding portion of the heavy chain variable region of an antibody;
- and
- (c) a peptide linker said first and second polypeptides (a) and (b) into said single chain polypeptide;
- said single chain polypeptide produced by the method comprising:
- (i) providing a genetic sequence coding for said single chain polypeptide;
- (ii) transforming a host cell with said sequence;
- (iii) expressing said sequence in said host;
- and
- (iv) recovering said single chain polypeptide having binding affinity for said given antigen.
A method of producing a genetic sequence coding for a single chain polypeptide having binding affinity for a given antigen, which comprises operably linking:
- (A) a genetic sequence coding for a first polypeptide comprising the antigen binding portion of a light chain variable region of an antibody to
- (B) a genetic sequence coding for a polypeptide comprising the antigen binding portion of a heavy chain variable region of an antibody to
- (C) a genetic sequence coding for a peptide linker suitable for linking said polypeptides in (A) and (B) into a single chain polypeptide;
- so as to provide said genetic sequence coding for said single chain polypeptide.
Dependencies are inferred from the claim text.