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US 5,258,041 AGranted
Method of biomolecule attachment to hydrophobic surfaces
AssigneeBio-Metric Systems, Inc.
InventorsPatrick Guire, Shawn G. Dunkirk
Life of this patent
Estimating the term…
Abstract
A method for attaching a biomolecule to a support having a hydrophobic surface, involving the use of a long chain chemical spacer having a hydrophobic guiding group capable of becoming embedded in the surface, and the biomolecule being covalently bound to the spacer at its opposite end.
Claims15
A method for attaching a biomolecule to a support having a hydrophobic surface, comprising:
- attaching, to the hydrophobic surface of the support, a spacer comprising a hydrophilic chemical chain carrying a relatively hydrophobic guiding group capable of becoming embedded in the hydrophobic surface, and covalently bonding a biomolecule to the spacer,
- wherein the spacer includes, along its length, a stopping group that is relatively hydrophilic to the guiding group and to the hydrophobic surface of the support, the stopping group being positioned between the bulk of the hydrophilic chain and the hydrophobic guiding group, and
- wherein the guiding group is an omega aminoundecanoic acid, an epsilon amino caproic acid, a gamma aminobutyric acid, or a beta-alanine.
A method for attaching a biomolecule to a support having a hydrophobic surface, comprising:
- (1) attaching, to the hydrophobic surface of the support a spacer comprising a hydrophilic chemical chain carrying a relatively hydrophobic guiding group capable of becoming embedded in the hydrophobic surface, and a stopping group, along the length of the spacer, the stopping group being relatively hydrophilic to the guiding group and to the hydrophobic surface of the support, the stopping group being positioned between the bulk of the hydrophilic chain and the hydrophobic guiding group, and
- (2) covalently bonding a biomolecule to the spacer, wherein the guiding group is derived from an aminoalkyl carboxylic acid.
A method for attaching a biomolecule to a support having a hydrophobic surface, comprising:
- (1) covalently bonding a biomolecule to a spacer, the spacer comprising a hydrophilic chemical chain carrying a relatively hydrophobic guiding group capable of becoming embedded in the hydrophobic surface, and a stopping group, along the length of the spacer, the stopping group being relatively hydrophilic to the guiding group and to the hydrophobic surface of the support, the stopping group being positioned between the bulk of the hydrophilic chain and the hydrophobic guiding group, and
- (2) attaching the spacer to the hydrophobic surface of the support, wherein the guiding group is derived from an aminoalkyl carboxylic acid.
Dependencies are inferred from the claim text.