Collagen constructs
AssigneeOrganogenesis, Inc.
InventorsPaul D. Kemp, Robert M. Carr, Jr., John G. Maresh
Life of this patent
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Abstract
The present invention provides collagen constructs and methods of making and using such constructs. The present invention also provides tissue equivalents having improved characteristics and methods of making and using such tissue equivalents. This invention also provides methods of producing highly concentrated solutions of collagen.
Claims34
A method of forming a construct comprising collagen, the method comprising the steps of:
- (a) providing an aqueous solution comprising collagen adjacent a permeable member, the permeable member being substantially impermeable to collagen and in contact with a concentrating agent, wherein the concentrating agent has an osmotic pressure higher than that of the collagen solution and permits selective mass transfer of solvent from the collagen solution through the permeable member;
- and
- (b) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the selective mass transfer of solvent from the collagen solution and the formation of the collagen construct at the permeable member.
A method of forming a multi-layer tubular collagen chamber, the interface between the inner and outer chamber comprising a tubular permeable member, the method comprising the steps of:
- (a) providing a first solution comprising collagen at a pH of about 2 to 4 to the outer chamber;
- (b) providing a concentrating agent to the inner chamber;
- (c) maintaining the first collagen solution, permeable member and concentrating agent under conditions sufficient to enable the tubular collagen construct to form at the permeable member;
- (d) replacing the first collagen solution with a second solution comprising collagen;
- and
- (e) maintaining the second collagen solution, permeable member and concentrating agent under conditions sufficient to enable a second tubular construct to form outwardly of the first tubular construct;
- and
- (f) repeating steps (d) and (e) if additional layers of tubular collagen construct are desired.
The method of 30, further comprising the step of causing fibrils to form in at least one layer of the collagen construct.
A method of forming a flat collagen construct by use of a device having an inner and outer container, the interface between the inner and outer chamber comprising a flat permeable member, the method comprising the steps of:
- (a) providing a solution comprising collagen to the inner chamber;
- (b) providing a concentrating agent to the outer chamber;
- and
- (c) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the flat construct to form at the permeable member.
A method of concentrating a solution of collagen comprising:
- (a) contacting a collagen solution with a permeable member, the permeable member being in contact with an agent for concentrating the collagen;
- (b) maintaining the collagen solution and concentrating agent under conditions sufficient to enable the collagen solution to reach a concentration of, from about 50 to 100 mg/ml.
Dependencies are inferred from the claim text.