Fusion proteins having a site for in vivo post-translation modification and methods of making and purifying them
AssigneesBiotechnology Research and Development Corporation, The Board of Trustees of the University of Illinois
InventorJohn E. Cronan, Jr.
Life of this patent
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Abstract
A hybrid DNA sequence encoding a fusion protein comprising: a first DNA sequence which encodes an amino acid sequence that allows for post-translation modification of the fusion protein; and a second DNA sequence joined end to end with the first DNA sequence and in the same reading frame, the second DNA sequence encoding a selected p The invention described herein was made in the course of work partially funded by Grant No. 2 RO1 AI15650 from the National Institutes of Health, U.S. Department of Health and Human Services. The U.S. government may have rights in this invention.
Claims9
A transformed host cell into which DNA has been introduced, or progeny of said transformed host cell, the introduced DNA comprising:
- (a) DNA coding for a fusion protein comprising:
- (i) a first DNA sequence which codes for a protein or polypeptide having an amino acid sequence that allows for post-translation biotination of the fusion protein;
- and
- (ii) a second DNA sequences joined end to end with the first DNA sequence and in the same reading frame, the second DNA sequence encoding a selected protein or polypeptide;
- and
- (b) DNA coding for biotin ligase;
- the DNA coding for the fusion protein and the DNA coding for biotin ligase being operatively linked to expression control sequences.
The host cell of claim 11 wherein the first DNA sequence codes for the 1.3S subunit of Propionibacterium shermanii transcarboxylase, tomato biotin protein, the alpha subunit of Klebsiella pneumoniae oxalacetate decarboxylase, Escherichia coli biotin carboxyl carrier protein or fragments of these proteins that allow for post-translation biotination of the fusion protein.
Dependencies are inferred from the claim text.