Process and reagent for determining the activity of chymotrypsin and trypsin in feces
AssigneeBoehringer Mannheim GmbH
InventorsGerald Moller, Klaus P. Kaspar
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Abstract
The present invention provides a process for the determination of the activity of chymotrypsin or trypsin in feces by measurement of the rate of fission of an appropriate substrate by a fecal suspension in an aqueous or aqueous-organic medium, wherein a fecal sample is suspended in the presence of a surface-active agent. The present invention also provides a reagent for carrying out this process, comprising a surface-active agent, an enzyme substrate and an aqueous salt solution, said reagent having a pH value of from 7 to 11.
Claims15
A process for the determination of chymotrypsin or trypsin in a feces sample, comprising the steps of:
- suspending the feces sample in an aqueous or aqueous-organic suspension medium including one or more water soluble salts in an amount whereby the ionic strength of the salts from 20 to 1000 mval/liter, and from 0.02% to 10% by weight of a surface-active agent selected from the group consisting of cationic and non-ionic tensides;
- contacting the suspended sample with a substrate specific for the determination of chymotrypsin or trypsin by measurement of the rate of fission of the substrate;
- and
- photometrically measuring the rate of fission of the substrate as a measure of the activity of chymotrypsin or trypsin in the suspension.
The process of claim 1, 31 or 32, wherein chymotrypsin is to be determined.
The process of claim 1, 31 or 32, wherein trypsin is determined.
Dependencies are inferred from the claim text.