Live attenuated newcastle disease virus vaccines and preparation thereof
AssigneeUniversity of Delaware
InventorJack Gelb, Jr.
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Abstract
Novel live, cold-adapted temperature-sensitive (CaTs), attenuated Newcastle disease virus vaccines are provided which are effectively immunogenic and contain a mutant of a Hitchner B.sub.1 parent strain of Newcastle disease virus.
Claims14
A mutant of the Hitchner B.sub.1 strain or Newcastle disease virus identified by microorganism accession number ATCC VR 2213, wherein the mutant virus is less able to replicate at 41.degree. C. than at 37.degree. C.
A method for making a live, cold-adapted, temperature-sensitive Newcastle disease virus vaccine which comprises cloning a Hitchner B.sub.1 Newcastle disease virus strain, serially passaging the cloned virus from 20 to 100 times through a culture medium at a lower than normal temperature of from 28 degrees C to 30 degrees C, and harvesting the resulting cold-adapted, temperature sensitive mutant.
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