Process for separation of .alpha.substituted carboxylic acid amides using l-amidase from Ochrobactrum anthropi
AssigneeDsm N.V.
InventorsTheodorus J. G. M. Van Dooren, Wilhelmus J. J. Van Den Tweel
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Abstract
Enzyme-catalysed preparation of an .alpha.-substituted optically active carboxylic acid, using enantioselective hydrolysis of a mixture of the enantiomers of the corresponding amide, an Ochrobactrum anthropi or an Klebsiella sp. being used as the enzyme. During the hydrolysis both the L-carboxylic acid and the remaining D-carboxylic acid amide are obtained with high optical purity and high yield, while the activity of the micro-organism is high. A large number of .alpha.-substituted carboxylic acid amides with a remarkable structural variety can be hydrolysed in this manner.
Claims7
A process for the preparation of an L-carboxylic acid or a D-carboxylic acid amide comprising:
- selectively hydrolyzing a mixture D,L-carboxylic acid amides where the .alpha.-carbon has a substituent selected from the group consisting of an amino, a hydroxyl and an N-hydroxylamine with Ochrobactrum anthropi NCIB 40321 or a L-amidase derived therefrom, and
- recovering the L-carboxylic acid or the D-carboxylic acid amide.
Dependencies are inferred from the claim text.