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US 5,245,012 AGranted
Method to achieve solubilization of spider silk proteins
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsStephen J. Lombari, David L. Kaplan
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Abstract
A recombinant spider silk protein can be obtained in a commercially useful form by cloning and the expression in a host cell of a polynucleotide encoding an endogenous spider silk protein or variant thereof. The sequencing of a spider silk protein is made possible by a method for solubilizing a spider silk protein.
Claims3
A method for solubilizing a spider silk protein or variant comprising the steps of:
- (A) providing a sample comprising at least one spider silk protein or variant;
- (B) contacting said sample with a solution consisting essentially of propionate and hydrochloric acid in a 50--50% volume per volume ratio;
- (C) solubilizing said spider silk protein or variant in said solution to obtain a solubilized spider silk protein or variant.
Dependencies are inferred from the claim text.