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US 5,244,798 AGranted
Reagent for determining triglycerides comprising a thermostable lipoprotein lipase from streptomyces
AssigneeUnitika Ltd.
InventorsAkira Takeda, Tomoko Kamei, Masao Kageyama, Kenzo Motosugi
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Abstract
A reagent composition for determining blood triglycerides comprising a color former and a thermostable lipoprotein lipase obtained from Streptomyces 7825 (FERM P-9983, FERM BP-2489) is presented.
Claims3
A reagent comprising a color former and a thermostable lipoprotein lipase for the quantitative determination of triglycerides in body fluids,
- wherein said thermostable liproprotein lipase has an activity to hydrolyze triglycerides in lipoproteins to glycerol and fatty acids, said activity being about 100% retained after treatment in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes;
- has a pH optimum of from about 8 to 9;
- has a temperature optimum of about 50.degree. C.;
- and
- wherein said thermostable lipoprotein lipase has a glycerol forming activity and a fatty acid forming activity, such that said glycerol forming activity is at least about 15% of said fatty acid forming activity.
2 more elements
Dependencies are inferred from the claim text.