Glycine anhydride dimethylol as a biocide and preservative

#1. Technical Field of the Invention

#This invention relates to the use of glycine anhydride dimethylol, as a biocide and an antimicrobial agent to kill microorganisms present in or on substances and to preserve a variety of substances susceptible to microbial contamination.

#2. Description of the Prior Art

#To further illustrate the various aspects of the invention, the following examples are provided. However, it is to be understood that their purpose is entirely illustrative and in no way intended to limit the scope of the invention. In the examples, all percentages are given by weight unless otherwise noted.

#To a stirred slurry of 11.4 g. (0.1 mole) of glycine anhydride in 41.0 ml. of water was added 16.2 g. (0.2 mole) of 37% aqueous formaldehyde. Addition of 0.10 g. of 10% aqueous sodium hydroxide solution caused the suspended solids to dissolve, followed by formation of a heavy white precipitate. The mixture was heated to 70.degree. C., by which time all solids dissolved. On cooling to room temperature, white crystals separated. The crystals were collected, washed with cold water, and dried in vacuum at 100.degree. C. for 1 hour. The white crystalline product, glycine anhydride dimethylol weighed 12.6 g. (72.4% yield), m.p. 185.degree.-188.degree. C. dec. Anal. Calcd. for C.sub.6 H.sub.10 N.sub.2 O.sub.4 (174.16): N, 16.09. Anal. Found: N, 1617.

#A. Minimum Inhibitory Concentration (MIC)