Fluorescent image densitometer of flying spot system
AssigneeShimadzu Corporation
InventorKazunari Sinya
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Abstract
A two-dimensional fluorescent densitometry for measuring a specimen plate having a specimen spot such as phospholipide two-dimensionally developed and fluorescent-labelled on a TLC plate. The densitometer comprises an excitation optical system for scanning a measuring plate with an excitation light beam, a fluorescence detection system for detecting a fluorescence from the measuring plate, a correction table for storing fluorescent data together with positional information in a scanning direction on the measuring plate, and an arithmetic section for dividing fluorescence detection data by fluorescent data at the same position in the scanning direction on the measuring plate stored in the correction table.
Claims3
A fluorescent image densitometer of flying spot system, comprising:
- an excitation optical system for scanning and irradiating a measuring plate with an excitation light beam;
- a fluorescence detection system for detecting a fluorescence from an excitation light irradiation position of the measuring plate;
- a plate uniformly applied with a fluorescent agent at least within a range of excitation light;
- a correction table for storing fluorescent data, measured with said plate mounted as the measuring plate, together with information of a position in a scanning direction on the measuring plate;
- and
- an arithmetic section for dividing fluorescence detection data measured with a specimen plate mounted as the measuring plate by fluorescent data at the same position in the scanning direction on the measuring plate stored in said correction table.
Dependencies are inferred from the claim text.