Method of reducing cellular immune response involving T-cells using CD8-bearing antigen presenting cells
AssigneeTKB Associates Limited Partnership
InventorsMark L. Tykocinski, David R. Kaplan
Life of this patent
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Abstract
Specific and nonspecific immunomodulation, enhancement of cellular engraftment, and modulation of nonimmune cells are achieved by using various membrane-binding and soluble CD8 compositions.
Claims7
A method for specifically reducing T cell proliferation or cytotoxicity, directed to alloantigens or processed antigens, comprising the steps of:
- (a) providing a non-naturally occurring antigen presenting cell which presents in, or on its surface the extracellular domain of CD8 and said alloantigens or processed antigens, and
- (b) exposing said T cells capable of responding to said alloantigens or processed antigens, for a time, and under conditions sufficient to reduce the specific cellular immune response of said cell T cells to said alloantigens or processed antigens.
A method of making a non-naturally occurring antigen presenting cell, which expresses the extracellular domain of CD8 on its surface, capable of specifically reducing T cell proliferation or cytotoxicity, directed to alloantigens or processed antigens, comprising the steps of:
- (a) isolating a eukaryotic cell comprising alloantigens to which a reduced cellular response is desired, and
- (b) transforming said cell with a gene encoding glycoinositolphospholipid-modified CD8, wherein said gene expresses glycoinositolphospholipid-modified CD8 in a manner which presents the extracellular domain of CD8 on the surface of said cell.
A method of making a non-naturally occurring antigen presenting cell, capable of specifically reducing T cell proliferation or cytotoxicity, directed to alloantigens or processed antigens, comprising the steps of:
- (a) isolating a cell comprising alloantigens or processed antigens, and
- (b) contacting said cell with a chimera for a time, and under conditions sufficient for binding of said chimera to the surface of said cell, wherein the chimera consists essentially of the extracellular domain of CD8 and a moiety not naturally associated with CD8, said moiety sufficient to bind said chimera to the surface of said cell in a manner which presents the extracellular domain of CD8 on said cell's surface.
A method of making a non-naturally occurring antigen presenting cell, capable of specifically reducing T cell proliferation or cytotoxicity, directed to alloantigens or processed antigens, comprising the steps of:
- (a) isolating a cell comprising alloantigens or processed antigens, and Fc receptors, and
- (b) contacting said cell with a chimera, consisting essentially of the extracellular domain of CD8 and an immunoglobulin Fc domain, for a time and under conditions sufficient for binding of said conjugate to the surface Fc receptors of said cell.
Dependencies are inferred from the claim text.