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US 5,240,837 AGranted
CDNAS encoding somatotropin, expression vectors and hosts
AssigneeThe Upjohn Company
InventorsChe-Shen C. Tomich, Eric R. Olson, John E. Mott
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Abstract
This invention discloses novel species of cDNAs encoding somatotropins, novel dual replicon vectors, hosts for such vectors and hosts for expressing the somatotropins encoded by such cDNAs.
Claims16
An expression plasmid capable of runaway at a constant temperature below 34.degree. C. which is useful for transforming a host cell and permitting the host cell to produce a heterologous protein, which plasmid comprises a mutant R1 replicon from pBEU-17, an orgin of replication from pBR322, and a cDNA encoding the heterologous protein.
E. coli host cell BST-1C (NRRL B-18303) or D112 (NRRL B-18168).
Dependencies are inferred from the claim text.