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US 5,236,840 AGranted
Method for growing crustacean virus in fish cells
AssigneeUniversity of Hawaii
InventorsPhilip C. Loh, Yuanan Lu
Life of this patent
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Abstract
A method is provided, whereby a crustacean virus is grown in fish cells. In particular, infectious hypodermal and hematopoietic necrosis virus (IHHNV) is grown in the established fish cell line, epithelioma papillosum cyprini (EPC).
Claims12
A method for growing crustacean virus comprising the steps of:
- introducing IHHNV virus particles into an established cell culture comprising epithelioma papillosum cyprini, derived from Cyprinus carpio;
- incubating said culture in a nutrient medium suitable for growth and replication of said virus;
- and
- harvesting said virus from said culture.
1 more element
Dependencies are inferred from the claim text.