Process for isolating nucleic acid
AssigneeAkzo N.V.
InventorsWillem R. Boom, Henriette M. A. Adriaanse, Tim Kievits, Peter F. Lens
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Abstract
The invention relates to a process, a combination of means for isolating nucleic acid from a nucleic acid-containing starting material and a testkit in order to amplify the nucleic acid obtained by said process. More in particular, the invention relates to a process and a kit for isolating nucleic acid from a nucleic acid-containing biological material such as whole blood, blood serum, urine, feces, cell cultures and the like.
Claims14
A process for isolating nucleic acid from a nucleic acid-containing starting material comprising mixing the starting material, a chaotropic substance and a nucleic acid binding solid phase, separating the solid phase with the nucleic acid bound thereto from the liquid, and washing the solid phase nucleic acid complexes.
A process to amplify captured nucleic acids comprising mixing the starting material, a chaotropic substance and a nucleic acid binding solid phase, separating the solid phase with the nucleic acid bound thereto from the liquid, washing the solid phase nucleic acid complexes and contacting the solid phase-nucleic acid complexes with a mixture comprising components that amplify the nucleic acid.
Dependencies are inferred from the claim text.