Vitamin D derivatives: therapeutic applications and applications to assays of metabolites of vitamin D

Abstract

Vitamin D derivatives corresponding to the following formula I ##STR1## in which R.sub.1 denotes a substituted alkyl group having 1 to 15 carbon atoms, in particular the side chains of vitamin D.sub.2 (C.sup.20 to C.sup.28) or D.sub.3 (C.sup.20 to C.sup.27), or these same chains partially modified, Y denotes H or OH or groups derived from the latter such as ester and ether; X denotes an alkyl chain, in particular of 1 to 6 carbon atoms, optionally substituted at different points by one or more functional group(s) an unsaturated alkyl chain having one or more carbon-carbon double or triple bond(s), it being possible for these chains, in addition, to bear functional groups, or an aromatic or heteroaromatic ring, optionally substituted with halogens, one (or more) hydroxyl, amine, formyl, carboxyl, thiol, cyano or nitro group(s), or alternatively groups derived from these latter, such as ether, ester, acetal or amide, or a halogen, a cyano, sulfoxide, sulfone, hydroxyl, thiol or amine group, or alternatively derivatives of these latter, such as ether, ester, amine amd hydrazine; R.sub.2 denotes a methyl group and R.sub.3 an H, or R.sub.2 is H and R.sub.3 is methyl, or R.sub.2 and R.sub.3 are H, or alternatively R.sub.2 and R.sub.3 together denote a methylene group=CH.sub.2. The present invention relates to the therapeutic application of these derivatives. The present invention also relates to a method for preparing these derivatives; and to an antigen prepared by the covalent binding of these derivatives with an immunogenic carrier protein and antibodies prepared using this antigen. Finally, the present invention also relates to tracers consisting of the conjugation of a derivative according to the invention with a labeling component such as an enzyme or an iodinated molecule, as well as to an assay method for metabolites of vitamin D comprising the use of these tracers as a reagent.

Dependencies are inferred from the claim text.