Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10)
AssigneeSchering Corporation
InventorsTimothy R. Mosmann, Kevin W. Moore, Martha W. Bond, Paulo J. M. Vieira
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Abstract
Mammalian genes and proteins, designated cytokine synthesis inhibitory factors (CSIFs) (now known as interleukin -10), are provided which are capable of inhibiting the synthesis of cytokines associated with delayed type hypersensitivity responses, and which, together with antagonists, are useful in treating diseases associated with cytokine imbalances, such as leishmaniasis and other parasitic infections, and certain immune disorders including MHC associated autoimmune diseases caused by excessive production of interferon-.gamma..
Claims16
An isolated nucleic acid capable of encoding a mature polypeptide of the open reading frame defined by the amino acid sequence:
- ##STR5##
An isolated nucleic acid capable of encoding a mature polypeptide defined by the amino acid sequence:
- ##STR7##
A nucleic acid capable of encoding with a continuous nucleotide sequence a polypeptide exhibiting cytokine synthesis inhibitory factor activity, said nucleic acid capable of forming a detectable hybrid with one or more probes derived from the cDNA inserts of pcD(SR.alpha.)-F115, pH5C, or pH15C, in the presence of 5X SET at 60.degree. C.
An expression vector capable of expressing in a host a mature polypeptide of the open reading frame defined by the amino acid sequence:
- ##STR8##
A method of producing a polypeptide exhibiting human cytokine synthesis inhibitory factor activity, the method comprising the steps of:
- constructing a vector comprising a nucleotide sequence coding for said polypeptide, wherein the nucleotide sequence is capable of being expressed by a host containing the vector and wherein the nucleotide sequence is selected from a group encoding mature polypeptides of the open reading frame defined by the amino acid sequence:
- ##STR11## incorporating the vector into the host;
- and maintaining the host in a culture medium under conditions suitable for expression of the nucleotide sequence into said polypeptide.
Dependencies are inferred from the claim text.