Method for the determination of plasminogen
AssigneeBehringwerke Aktiengesellschaft
InventorHans-Jurgen Kolde
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Abstract
A method for the determination of plasminogen in body fluids in the presence of streptokinase using a substrate for plasmin, the action of plasmin on which results in a cleavage product which can be measured optically, which method comprises essentially simultaneous addition of streptokinase and chromogenic or fluorogenic substrate for plasmin to the body fluid, and determination of the amount of the cleavage product formed in a defined time, or of the rate of formation of the cleavage product, and from this the concentration of plasminogen, is described. When the amount of streptokinase which is used is such that the plasminogen present is in molar excess, this method can be used to determine the rate of activation of plasminogen.
Claims5
A method for determining the concentration of plasminogen in plasma which comprises the steps of:
- (1) simultaneously adding streptokinase and a chromogenic or fluorogenic peptide substrate for plasmin to the plasma;
- and
- (2) measuring either the amount of cleavage product formed in a defined time or the rate of formation of cleavage product to determine the concentration of plasminogen in the plasma.
A method for determining the rate of activation of plasminogen in plasma which comprises the steps of:
- (1) simultaneously adding streptokinase and a chromogenic or fluorogenic peptide substrate for plasmin to the plasma;
- and
- (2) measuring either the amount of cleavage product formed in a defined time or the rate of formation of cleavage product to determine the rate of activation of plasminogen in the plasma.
Dependencies are inferred from the claim text.