Methods of detecting the presence of human herpesvirus-7 infection
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorNiza Frenkel
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Abstract
The present invention relates to a new human herpesvirus-7, proteins encoded in the genome of the virus, and antibodies specific for the virus and proteins. The virus was isolated from human peripheral blood mononuclear cells following incubation of the cells under conditions promoting T cell activation. Cultures of lymphocytes infected with the virus exhibited a cytopathic effect and electron microscopic analyses revealed a characteristic herpesvirus structure. The new virus is distinct from previously characterized human herpesviruses. The invention also relates to bioassays for the diagnosis of human herpesvirus-7 and the detection of human herpesvirus-7 in a biological sample. It further relates to a vaccine for humans against human herpesvirus-7.
Claims26
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of:
- ii) coating a substrate surface with a human herpesvirus-7 structural antigen
- ii) contacting said surface with serum suspected of containing human herpesvirus-7 specific antibodies from said human patient and
- iii) detecting the presence or absence of a complex formed between said antigen and antibodies specific thereof, if present in said serum, wherein the presence of said complex indicates the presence of said infection.
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of:
- i) coating a surface with antibodies a human herpesvirus-7 structural antigen;
- ii) obtaining a biological sample from said patient;
- iii) contacting said surface with said sample;
- and
- iv) detecting the presence or absence of a complex formed between said antibodies and virus, if present in said sample, wherein the presence of said complex indicates the presence of said infection in said patient.
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of:
- obtaining a sample of serum suspected of containing herpesvirus-7 specific antibodies from said patient;
- contacting said sample of serum with a human herpesvirus-7 structural antigen;
- and
- determining if specific binding has occurred between said antigen and the antibodies, if contained in said serum sample,
- wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of:
- obtaining a biological sample from said patient;
- contacting said biological sample with an antibody specific to a human herpesvirus-7 structural antigen;
- determining if specific binding has occurred between said antibody and said antigen present in said sample,
- wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
A monoclonal antibody specific for a human herpesvirus-7 structural antigen, but not for other human herpesviruses.
An isolated antibody that specifically binds to a human herpesvirus-7 structural antigen, but not to any other herpesviruses.
Dependencies are inferred from the claim text.