Smooth muscle mitogen
AssigneeChildren's Medical Center Corporation
InventorsMoses J. Folkman, Yuen Shing
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Abstract
A novel growth factor (BTC-CG) was purified from the conditioned medium of pancreatic beta tumor cells initially derived from transgenic mice (RIPl-Tag2). The purification scheme included BioRex 70 chromatography, phenyl-Sepharose chromatography, TSL-GEL heparin FPLC and C4 reverse phase HPLC. The peptide also stimulated proliferation of bovine smooth muscle cells. It was not inactivated by boiling, by 10 mM dithiothreitol or by exposure to IM acetic acid. Biological activity of BTC-GF was recovered from a single band of protein which had a molecular weight of 2,000 on SDS-PAGE. The Partial N-terminal amino acid sequence of this protein was determined with an ABI 470A protein sequencer as: Asp-Gly-[X]-Thr-[X]-Arg-Thr-Pro-Glu-[X]-Asn-Gly.
Claims10
A purified protein having an N-terminal amino acid sequence comprising:
- SEQ 1D No:1, wherein said protein stimulated proliferation of smooth muscle cells.
A purified polypeptide which includes the amino acid sequence of SEO ID NO:1.
Dependencies are inferred from the claim text.