In-vitro method for producing antigen-specific human monoclonal antibodies
AssigneeAkzo N.V.
InventorDiana K. Goroff
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Abstract
A method of amplifying production of IgG, IgA and IgM antigen specific human monoclonal antibodies from transformed, T-cell depleted, human peripheral blood lymphocytes through the use of an adjuvant system consisting of 8-mercaptoguanosine and at least one of the cytokines interleukin-4 and interleukin-6. Also included in the invention is the adjuvant system and a kit comprising the system.
Claims8
A method for amplifying production of antigen specific human IgG and IgA monoclonal antibodies comprising
- a) obtaining human peripheral blood lymphocytes (PBL),
- b) depleting T-cells from said PBL,
- c) transforming the T-cell depleted PBL with a transforming agent in the presence of antigen and the adjuvants 8-mercaptoguanosine and at least one selected from the group consisting of IL-4 and IL-6,
- d) identifying cells producing antigen specific IgG and IgA antibodies, and
- e) cloning said identified cells.
A method for amplifying production of antigen specific human IgM monoclonal antibodies comprising
- a) obtaining human peripheral blood lymphocytes,
- b) depleting T-cells from said PBL,
- c) transforming the T-cell depleted PBL with a transforming agent in the presence of antigen and the adjuvants 8-mercaptoguanosine and at least one selected from the group consisting of IL-4 and IL-6,
- d) identifying cells producing antigen specific IgM antibodies, and
- e) cloning said identified cells.
A method for amplifying production of human immunoglobulins comprising
- a) obtaining human peripheral blood lymphocytes,
- b) depleting T-cells from said PBL,
- c) transforming the T-cell depleted PBL with a transforming agent in the presence of 8-mercaptoguanosine at least one of the adjuvants selected from the group consisting of IL-6 and IL-4,and
- d) culturing the transformed PBL in the presence of 8-mercaptoguanosine and at least one of IL-4 or IL-6, thereby producing immunoglobulins.
Dependencies are inferred from the claim text.