Method for assessing lung maturity using fluorescence from naphthalene-based probes
AssigneeUniversity of Hawaii
InventorsDavid M. Jameson, Nadhipuram V. Bhagavan
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Abstract
A method for assessing lung maturity in a fetus or an infant comprises adding a fluorescent dye to a pulmonary effluent, causing the dye to fluoresce by illumination with an excitation wavelength of monochromatic light, and measuring the intensity of the fluorescence at two different wavelengths. Alternatively, the method comprises sequential illumination with two different excitation wavelengths and measurement of the intensity of fluorescence caused by each excitation wavelength at a single emission wavelength.
Claims10
A method for assessing lung maturity in a patient comprising
- (a) adding a naphthalene-based fluorophore to a sample comprising lipids, said lipids originating from at least one lung of said patient,
- (b) illuminating said sample containing said fluorophore with monochromatic light of wavelength A to cause fluorescence,
- (c) measuring the intensity of emission of said fluorescence at wavelength B and at wavelength C to obtain the intensity values I.sub.B and I.sub.C and
- (d) using said values I.sub.B and I.sub.C to assess lung maturity wherein wavelength A is selected from 320 to 440 nm, wavelength B corresponds to the emission wavelength which would yield about the maximum intensity if the lipid composition were in its most viscous state and wavelength C corresponds to the emission wavelength which would yield about the maximum intensity if the lipid composition were in its least viscous state.
A method for assessing lung maturity in a patient comprising
- (a) adding a naphthalene-based fluorophore to a sample comprising lipids, said lipids originating from at least one lung of said patient,
- (b) illuminating said sample containing said fluorophore with monochromatic light of wavelength A to cause fluorescence,
- (c) measuring the intensity at wavelength B of emission of said fluorescence caused by step (b) to obtain the intensity value I.sub.A
- (d) illuminating said sample containing said fluorophore with monochromatic light of wavelength C to cause fluorescence,
- (e) measuring the intensity at wavelength B of emission of said fluorescence caused by step (d) to obtain the intensity value I.sub.C and
- (f) using said values I.sub.A and I.sub.C to assess lung maturity wherein said wavelength A is selected from 320 to 380 nm, wavelength B is selected from 460 to 540 nm and wavelength C is selected from 380 to 440 nm.
Dependencies are inferred from the claim text.