Methods for producing hybrid phospholipid-binding proteins
AssigneeZymoGenetics, Inc.
InventorDonald C. Foster
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Abstract
Methods are disclosed for producing hybrid phospholipid-binding proteins from eukaryotic cells. DNA constructs comprising a transcriptional promoter, at least one signal sequence and a hybrid phospholipid-binding protein coding sequence comprising at least one lipocortin lipid-binding domain joined to a gla-domainless, vitamin K-dependent protein and a transcriptional terminator are also disclosed.
Claims5
A hybrid phospholipid-binding protein comprising at least one lipocortin phospholipid-binding domain joined to a gla-domainless, vitamin K-dependent protein, wherein said hybrid phospholipid-binding protein has substantially the same biological activity as the active form of the vitamin K-dependent protein.
A hybrid phospholipid-binding protein having substantially the same biological activity as human protein C or human activated protein C said protein comprising at least one lipocortin phospholipid-binding domain joined to a gla-domainless activated protein C.
A hybrid phospholipid-binding protein having substantially the same biological activity as human protein C or human activated protein C, said protein comprising the amino acid sequence of FIG. 7 from amino acid 1 through amino acid 136 joined to the amino acid sequence of FIG. 2 encoding amino acid 46 through amino acid 419.
Dependencies are inferred from the claim text.