Culture bag

A culture bag which comprises an integrally formed culture room and medium storage room, and at least one each of cell injection opening and medium discharge opening formed in the culture room and a medium injection opening formed in the medium storage room, with the culture room and medium storage room communicating with each other through a passage, said culture bag being formed by fusion-bonding the peripheries of two sheets for the storage room and one sheet for the culture room in such a manner that the two sheets for the storage room face to each other to form the medium storage room and one of the two sheets for the storage room and the one sheet for the culture room face to each other to form the culture room, said sheets for the storage room being made of a resin composition composed of 100 parts by weight of vinyl chloride homopolymer or copolymer and 30-150 parts by weight of ethylene-vinyl acetate-carbon monoxide copolymer, and having a gas permeability of 100-350 ml/m.sup.2.24hr.atm to oxygen and a gas permeability of 200-1000 ml/m.sup.2.24hr.atm to carbon dioxide, said sheet for the culture room being made of a resin composition composed of 100 parts by weight of vinyl chloride homopolymer or copolymer and 150-260 parts by weight of ethylene-n-butyl acrylate-carbon monoxide copolymer, and having a gas permeability of 600-3000 ml/m.sup.2.24hr.atm to oxygen and a gas permeability of 1000-30,000 ml/m.sup.2.24hr.atm to carbon dioxide.