Diagnostic kit and diagnostic method utilizing carbohydrate receptors
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsVictor Ginsburg, Howard C. Krivan, David D. Roberts
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Abstract
A diagnostic kit for detecting the presence of microorganisms, comprising an insoluble substrate; and a carbohydrate receptor immobilized on the insoluble substrate, the carbohydrate receptor being capable of adsorbing microorganisms; and a labelled reagent useful for detecting the presence of microorganisms bound to the carbohydrate receptors and a method for detecting the presence of specified microorganisms in a sample, which comprises contacting a sample to be tested with carbohydrate receptors immobilized on an insoluble substrate; and determining the extent of binding of microorganisms in the sample to the carbohydrate receptors by use of a labelled reagent.
Claims16
A method for detecting the presence of Mycoplasma pneumoniae in a sample which comprises:
- contacting said sample with a carbohydrate receptor immobilized on an insoluble support, said carbohydrate receptor being a glycolipid selected from the group consisting of Gal(3SO.sub.4).beta.1-1Cer, Gal(6SO.sub.4).beta.1-1Cer, Gal(3SO.sub.4).beta.1-4Glc.beta.1-1Cer, Gal(3SO.sub.4).beta.1-3alkylaclglycerol, GlcNAc.beta.1-3Gal.beta.1-4Glc.beta.1- 1Cer and Gal.beta.1-4GlcNAc.beta.1-3Gal.beta.1-4Glc.beta.1-1Cer, for a time and under conditions sufficient for Mycoplasma pneumoniae present in said sample to bind to said carbohydrate receptor thereby forming a complex on said support;
- adding a labelled reagent to said support wherein said labelled reagent binds to the complex;
- and
- detecting the presence of the labelled reagent bound to said support as a means of detecting the presence of Mycoplasma pneumoniae in said sample.
A method of detecting the presence of a microorganism in a sample wherein said microorganism is selected from the group consisting of Streptococcus pneumoniae, Hemophilus influenzae, Pseudomonas aeruginosa, Pseudomonas cepacia and Pseudomonas maltophilia, which comprises:
- contacting said sample with a carbohydrate receptor immobilized on an insoluble support, said carbohydrate receptor being a glycolipid selected from the group consisting of GalNAc.beta.1-4Gal.beta.1-4Glc.beta.1-1Cer, Gal.beta.1-3GalNAc.beta.1-4Gal.beta.1-4Glc.beta.1-1Cer and Fuc.alpha.1-2Gal.beta.1-3GalNAc.beta.1-4Gal.beta.1-4Glc.beta.1-1Cer, for a time and under conditions sufficient for said microorganism present in said sample to bind said carbohydrate receptor thereby forming a complex on said support;
- adding a labelled reagent to said support wherein said labelled reagent binds to the complex;
- and
- detecting the presence of the labelled reagent bound to said support as a means of detecting the presence of said microorganism in said sample.
Dependencies are inferred from the claim text.