Recombinant DNA molecule comprising lymphocyte function-associated antigen 3 phosphatidylinositol linkage signal sequence
AssigneeBiogen, Inc.
InventorBarbara P. Wallner
Life of this patent
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Abstract
DNA sequences derived from a phosphatidylinositol-linked form of lymphocyte function-associated antigen 3 ("LFA-3") are provided which code for a phosphatidylinositol linkage signalling sequence. The linkage signalling sequence may be linked to DNA coding for secretory proteins or polypeptides to obtain phosphatidylinositol-linked chimeric proteins or polypeptides. The chimeric proteins can be used to produce targeted drugs, to form micellular or liposomal drug delivery systems, or to improve the purification or screening of particular cells, proteins or DNA libraries.
Claims15
A hybrid DNA molecule comprising (a) a first DNA molecule coding for a secreted protein, a portion of a secreted protein or an extracellular region of a membrane protein, and, downstream of said first DNA molecule, (b) a second DNA molecule coding for a lymphocyte function-associated antigen 3 phosphatidylinositol linkage signalling sequence operatively linked to said first DNA molecule such that expression of the hybrid DNA molecule results in a phosphatidylinositol-linked polypeptide.
A recombinant DNA molecule comprising (a) a first DNA molecule coding for a secreted protein, a portion of a secreted protein or an extracellular region of a membrane protein, (b) a second DNA molecule downstream of said first DNA molecule coding for a lymphocyte function-associated antigen 3 phosphatidylinositol linkage signalling sequence and operatively linked to said first DNA molecule such that expression of the first and second DNA sequences results in a phosphatidylinositol-linked polypeptide, and (c) an expression control sequence operatively linked to the first DNA molecule.
A eukaryotic host cell culture transfected with a recombinant DNA molecules comprising (a) a first DNA molecule coding for a secreted protein, a portion of a secreted protein or an extracellular region of a membrane protein, (b) a second DNA molecule downstream of said first DNA molecule coding for a lymphocyte function-associated antigen 3 phosphatidylinositol linkage signalling sequence and operatively linked to said first DNA molecule such that expression of the first and second DNA sequences results in a phosphatidylinositol-linked polypeptide, and (c) an expression control sequence operatively linked to the first DNA molecule.
Dependencies are inferred from the claim text.