Monoclonal antibodies against glycated albumin, hybrid cell lines producing these antibodies, and use therefore

A monoclonal antibody specifically binds the glycated albumin epitope specifically bound by the monoclonal antibody produced by cell line ATCC HB 9596.

A method of quantitating glycated albumin which comprises:

• contacting a sample suspected of containing glycated albumin with a diagnostically effective amount of a monoclonal antibody, or fragment thereof, said monoclonal antibody or fragment thereof specifically binds an epitope which is found on native glycated albumin which has been subjected to no artificial modification but which is not found on unglycated albumin, said epitope occurring at both lys-lys and lys-his sequences, said antibody specifically bound more than one of said sequences located at a residue selected from the group consisting of lysine-199, lysine-281, lysine-439, and lysine-525, and said antibody being insignificantly reactive with other proteins;
• and
• quantitating monoclonal antibody or fragment thereof bound to components of the sample, amounts of bound monoclonal antibody or fragment thereof correlating with amounts of glycated albumin in the sample.

A monoclonal antibody which specifically binds an epitope which is found on native glycated albumin which has been subjected to no artificial modification but which is not found on unglycated albumin, said epitope occurring at both lys-lys and lys-his sequences, said antibody specifically binding more than one of said sequences located at a residue selected from the group consisting of lysine-199, lysine-281, lysine-439, and lysine-525, and said antibody being insignificantly reactive with other proteins.