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AssigneesBoyce Thompson Institute for Plant Research, Inc., Texas A&M University
InventorsRoman P. Legocki, Misuk Legocki, Aladar A. Szalay, Thomas O. Baldwin
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A host microorganism is genetically and stably modified by the insertion into any of its non-essential chromosomal location of a non-homologous, recombinant foreign DNA fragment, maintaining an insertion of a luxAB gene of a selected bioluminescent bacterium such as V. harveyi, such that the expression of the luxAB genes causes the production of a luciferase enzyme which, in turn, catalyzes a light-emitting reaction in the presence of the appropriate substrate. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism, each with a known and related cell (or plasmid) count.
An aerobic or facultative anaerobic microoganism which is capable of homologous recombination, transformed by the integration of an isolated foreign DNA fragment, within any of its chromosomal regions, wherein integration of transforming DNA does not alter cell viability, said foreign DNA fragment comprising lux AB genes of any vibrio bioluminescent bacterium fused in operable linkage with an active promoter, and further comprising an antibiotic resistance marker, wherein said active promoter is selected from the group consisting of constitutive promoters of gene expression and inducible promoters of gene expression, and is active in the transformant.
The plasmid vector pFIT001 as deposited at Agriculture Research Service under accession number NRRL B-18080.
The plasmid vector pPALE001 as deposited at Agriculture Research Service under accession number NRRL B-18082.
The plasmid vector pMR19 as deposited at Agriculture Research Service under accession number NRRL B-18081.
A method of stably modifying a microorganism using bacterial luciferase genes lux AB from any vibrio bioluminescent bacteria, comprising the steps of:
A method of stably modifying a microorganism using bacterial luciferase genes lux AB from any vibrio bioluminescent bacterium, comprising the steps of:
An aerobic or facultative anaerobic microorganism which is capable of homologous recombination, transformed by the integration of an isolated foreign DNA fragment, within any of its chromosomal regions, wherein integration of transforming DNA does not alter cell viability, said foreign DNA fragment comprising lux AB genes of any vibrio bioluminescent bacterium fused in operable linkage with an active promoter, wherein said active promoter is selected from the group consisting of constitutive promoters of gene expression and inducible promoters of gene expression, and is active in the transformant.
Dependencies are inferred from the claim text.